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. 2010 Aug 4;99(3):961–970. doi: 10.1016/j.bpj.2010.04.063

Figure 6.

Figure 6

Application of γ normalization to protein samples. Two different PSD-95 mutants are shown: PSD20 contained the mutations S142C-Y236C (open); PSD21 contained the mutations E135C-Y236C (shaded). Proteins were randomly labeled with a mixture of Alexa 555 and Alexa 647. Data shown was measured under optical path 1. (A) Histogram of EPR for PSD20 (open) and PSD21 (shaded). Data points are shown as solid circles with the Gaussian fit shown as a solid line. (B) Histogram of γ-values compiled from individual photobleaching events. (C) Histograms of FRET efficiency normalized with γIndividual. Because of differences in γ, samples with similar FRET display differing EPR.