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. 2010 Aug 4;99(3):961–970. doi: 10.1016/j.bpj.2010.04.063

Table 1.

Wavelength cutoffs of the optical elements in the microscopy filter sets

Optical path Long pass filter Dichroic mirror cutoff Bandpass filter (donor) Bandpass filter (acceptor) EMCCD camera Theoretical ηA/D Empirical ηA/D
1 550 nm 593 nm 562/40 670/30 Andor iXON 1.41 0.79
2 None 645 nm 585/70 700/75 Cascade 512B 0.78 N/D
3 550 nm 645 nm 585/70 670/30 Andor iXON 1.08 0.46