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Effect of γ-normalization methodology on the convergence of DNA FRET values recorded under different optical paths
| Normalization method | Average standard deviation of the mean |
|---|---|
| Raw | 0.0724 |
| ηA/D (Theoretical) | 0.0470 |
| ηA/D (Empirical) | 0.0340 |
| γEmpirical | 0.0362 |
| Universal γPhotobleach | 0.0220 |
| Global γPhotobleach | 0.0198 |
| Individual γPhotobleach | 0.0082 |
