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. 2010 Aug 4;99(3):897–904. doi: 10.1016/j.bpj.2010.04.070

Figure 1.

Figure 1

The interaction between BRIa and the CK2 subunit β is dependent upon BMP2. (Upper) Control and BMP2-stimulated cells were coimmunoprecipitated for BRIa and blotted for the CK2 β-subunit. The appearance of a band indicated an association between BRIa and the CK2 β-subunit. The addition of BMP2 led to a decrease of BRIa-CK2 interaction. As a negative control, blocking peptides (BP) were added during the IP to block the pull-down of BRIa. There was no CK2 β-subunit band with the blocking of BRIa pull-down. As for positive controls, blots were stripped and reprobed for the IP protein. (Lower) The reciprocal of the upper blot. The blots shown are representative.