Figure 2.

Peptides block the interaction between BRIa and CK2. (A) Cells were stimulated with the control peptide (HD) or designed peptides CK2.1, CK2.2, and CK2.3, followed by lysis, normalization of protein content, and IP with the CK2 β-subunit antibody. Western blots were probed for BRIa, then stripped and reprobed for CK2 β-subunit as an IP control. The addition of designed peptides led to a decrease in band intensity. Reciprocal IP is shown in the lower panel. The blots shown are representative. (B) Cells stimulated with peptides were fixed and dual-labeled for BRIa and the CK2 β-subunit, as described earlier. The addition of peptides resulted in a reduced energy transfer between BRIa and CK2-β. The error bars depict the SE of five independent experiments (p < 0.05).