Figure 6.

Mineralization is induced by blocking the BRIa-CK2 interaction. C2C12 cells were incubated with BMP2 or 100 nM peptides. Cells were fixed and subjected to von Kossa staining for mineralization. (A) Addition of BMP2 led to a significant increase in the area of mineralization (p < 0.05). The addition of peptides increased mineralization in a manner equivalent to that seen with BMP2 stimulation. Error bars depict the SE. (B) A representative low- and high-magnification light field image of the mineralization.