Table 2.
Keq values measured for rhodopsin reconstituted into membranes of various composition (lipid/rhodopsin molar ratio = 250/1, temperature 37°C, pH 7.0)
| Lipid 1 | Lipid 2 | Mole fractionof lipid 2 | Keq |
|---|---|---|---|
| POPC | N/A | N/A | 2.18 ± 0.15 |
| POPC | DOPC | 0.5 | 2.84 ± 0.16 |
| POPC | DOPC | 0.625 | 2.65 ± 0.2 |
| POPC | DOPC | 0.75 | 3.11 ± 0.19 |
| N/A | DOPC | 1 | 3.2 ± 0.18 |
| POPC | DOPE-Me2 | 0.5 | 3.4 ± 0.2 |
| POPC | DOPE-Me2 | 0.625 | 4.07 ± 0.2 |
| POPC | DOPE-Me2 | 0.75 | 5.25 ± 0.29 |
| N/A | DOPE-Me2 | 1 | 6.35 ± 0.2 |
| POPC | DOPE-Me1 | 0.5 | 3.56 ± 0.15 |
| POPC | DOPE-Me1 | 0.625 | 4.61 ± 0.18 |
| POPC | DOPE-Me1 | 0.75 | 5.62 ± 0.2 |
| N/A | DOPE-Me1 | 1 | 7.24 ± 0.23 |
| POPC | DOPE | 0.5 | 4.1 ± 0.19 |
| POPC | DOPE | 0.625 | 5.33 ± 0.23 |
| POPC | DOPE | 0.75 | 7 ± 0.15 |
| POPC | SDPE | 0.125 | 1.51 ± 0.12 |
| POPC | SDPE | 0.25 | 3.66 ± 0.2 |
| POPC | SDPE | 0.5 | 6.11 ± 0.25 |
| POPC | SDPE | 0.75 | 7.53 ± 0.27 |
| POPC | SDPC | 0.5 | 2.97 ± 0.17 |
| N/A | SDPC | 1 | 4.3 ± 0.25 |