Figure 8.
Treatment with 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) enhanced lung peroxisome proliferator activated receptor γ (PPARγ) expression and suppressed pulmonary injury caused by hyperoxia in Nrf2+/+ mice. (A) Bronchopulmonary lavage (BAL) analysis determined the concentration of total proteins and the number of lung neutrophils in Nrf2+/+ and Nrf2−/− mice pretreated with either vehicle or 15d-PGJ2 followed by air and O2 exposure (48 and 72 h). Data are presented as group mean ± SEM (n = 3–7/group). * = significantly higher than genotype- and treatment-matched air controls (P < 0.05). + = significantly lower than genotype- and exposure-matched vehicle-treated mice (P < 0.05). (B) Aliquots of lung total and nuclear proteins were subjected for Western blotting to determine PPARγ levels in Nrf2+/+ and Nrf2−/− mice treated with vehicle or 15d-PGJ2 after air or O2 exposure (72 h). Representative images from multiple analysis (n = 3 for total proteins, n = 2 for nuclear proteins) are presented.