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. Author manuscript; available in PMC: 2010 Aug 2.
Published in final edited form as: Oncogene. 2004 Feb 12;23(6):1239–1247. doi: 10.1038/sj.onc.1207205

Figure 3.

Figure 3

PTP-specific inhibitors prevent As2O3-induced PTP opening and cyto c release, (a, b) PTP opening was monitored as described in ‘Materials and methods’. Isolated mitochondria were pretreated with 5 µm CsA, or 50 µm BA (c) or preincubated with different concentrations of Bcl-XL as indicated (d) in 50 µl PT buffer for 5 min before As2O3 (5 µm) being added, and further incubated at 25°C for 60 min. A volume of 100 µm CaCl2 was added at the end of experiments as a positive control for PTP opening. Released cyto c was detected as described in ‘Materials and methods’. Data are representative of at least three independent experiments