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. Author manuscript; available in PMC: 2010 Aug 2.
Published in final edited form as: Neuroscience. 2007 Apr 30;146(4):1677–1688. doi: 10.1016/j.neuroscience.2007.02.064

Figure 5. Colocalization of GAD 67 and 5-HT2CR in layer IV of the PrL.

Figure 5

Photomicrographs of double-label immunofluorescent staining for the 5-HT2CR (green, A), and GAD 67 (red, B). Figure C displays the overlay of images in A and B to demonstrate colocalization. Approximately 50% of 5-HT2CR-positive cells in the field also contain GAD 67-IR. Examples of cells that express immunoreactivity for GAD 67 and 5-HT2CR are shown by arrows. D. Schematic drawing adapted from Paxinos and Watson (2004) corresponding to coronal sections (bregma 3.24 mm) from which images in A–C were captured. Scale bar: 50 μm. ACC: anterior cingulate cortex; PrL: prelimbic cortex; IL: infralimbic cortex.