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. 2010 Jul 15;460(1-2):20–29. doi: 10.1016/j.gene.2010.03.015

Fig. 5.

Fig. 5

Chromatin immunoprecipitation analysis of RFX1 and RFX2 binding to the ALMS1 proximal promoter. PANC-1 cells were incubated in growth medium containing 0.5% FCS for 48 h prior to analysis. (A,B) qPCR analysis of anti-RFX1 (A), anti-RFX2 (B) and IgG ChIP fractions with a Taqman assay encompassing the evolutionarily conserved X-box within the ALMS1 promoter. Note the differing scales of the y-axes. (C) qPCR analysis of anti-RFX1, anti-RFX2 and IgG ChIP fractions with a Taqman assay targeting a negative control region upstream of GAPDH. ChIP assays were performed with 2 or 4 µg of anti-RFX1, and with 2, 4 or 8 µg of anti-RFX2 and IgG, as indicated. DNA recovery is expressed as percentage of input (mean ± s.d. from triplicate qPCR assays).