Figure 2.
Tumour necrosis factor-related apoptosis inducing ligand (TRAIL) signalling in monocyte-derived dendritic cells (DCs) is mediated by TRAIL-R2. (a) Western blot analysis of TRAIL receptor expression in monocytes, immature DCs (iDCs) and DCs stimulated with lipopolysaccharide (LPS) for 20 hr. The arrows indicate the TRAIL receptors. The asterisks indicate the glycosylated receptors.63 (b) Fluorescence-activated cell sorting analyses of cell surface TRAIL receptor expression in day 8 iDCs or LPS-treated DCs. Tinted curves represented staining with isotype control immunoglobulin G. Percentages of cells in the positive gates are shown. (c) Confocal microscopy of TRAIL-R2 localization in untreated iDCs and LPS-treated DCs. Red: TRAIL-R2; Green: Mitotracker Red; Blue: Hoescht 33342.