Skip to main content
. 2010 Aug;130(4):504–515. doi: 10.1111/j.1365-2567.2010.03266.x

Figure 3.

Figure 3

Neutralization tumour necrosis factor-related apoptosis inducing ligand (TRAIL) partially inhibited the expression of co-stimulation markers. (a) Effect of anti-TRAIL neutralizing antibody on lipopolysaccharide (LPS) -induced TRAIL expression. Immature dendritic cells (iDCs) were stimulated as indicated for 20 hr. Bioactive TRAIL in the culture supernatants was determined by enzyme-linked immunsorbent assay (ELISA; mean ± SEM). The samples treated with LPS and control immunoglobulin G (IgG) were normalized as 100%. *P < 0·0001. (b) Effect of tumour necrosis factor (TNF) neutralizing antibody on LPS-induced TNF expression. Production of TNF in culture supernatants was measured by ELISA as in (a) (mean ± SEM). *P < 0·0001. (c–g) TRAIL neutralization moderately inhibited up-regulation of co-stimulatory molecules on DCs. Monocyte-derived iDCs were left untreated, stimulated with LPS + control murine IgG, LPS + anti-TRAIL antibody or LPS + anti-TNF antibody for 24 hr. The expression of (c) CD80, (d) CD83, (e) CD86, (f) HLA-ABC and (g) HLA-DR was examined by flow cytometry. The percentages of cells in the positive gates and the mean fluorescence intensities (in parentheses) are shown.