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. 2010 Aug;130(4):545–555. doi: 10.1111/j.1365-2567.2010.03253.x

Figure 1.

Figure 1

Immortalized porcine aortic endothelial cells (PAEC), MPN3 cells, retained sensitivity to xenogeneic natural killer (NK) -mediated lysis. (a) A standard 51Cr-release assay was performed using interleukin-2 (IL-2) -activated human primary NK cells as effector cells and MPN3 cells and primary PAEC as target cells. Specific lysis (%) was presented as mean + SD obtained from three to six replicates. The data shown are representatives of three independent experiments. (b) A standard 51Cr-release assay was performed with IL-2-activated human primary NK cells as effector cells and MPN3 as target cells at an effector to target (E : T) ratio of 3 : 1. NK cells were pre-incubated with 10 μg/ml designated blocking monoclonal antibodies (mAbs) or with their appropriate isotype control. Specific lysis (%) was presented as mean + SD of triplicates. The data are representatives of minimum three independent experiments. Students’t-test was performed. *P < 0·05.