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. 2010 Aug;130(4):597–607. doi: 10.1111/j.1365-2567.2010.03264.x

Figure 4.

Figure 4

Effect of type I interferons (IFNs) on dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM3) grabbing nonintegrin (DC-SIGN) expression on breast milk macrophages (BrMMø). BrMMø co-cultured with IFN for 5 days are shown as thick lines and isotype controls as dotted lines (a). Both type I IFNs, particularly IFN-β (lower panels), showed a strong capacity to suppress the enhancement of DC-SIGN expression in BrMMø cultured with IFN for 5 days in the presence of 20 ng/ml IL-4 (indicated as thin lines), and this suppression was dose-dependent. As controls (the two left-hand panels), DC-SIGN expression in BrMMø cultured for 5 days in medium alone is shown as shaded lines, that for isotype controls as dotted lines, and that for IFN-treated BrMMø as thick lines. (b) Dot-plot analyses for BrMMø co-cultured with IFNs and IL-4. The effect of IFNs on DC-SIGN expression on BrMMø co-cultured with IL-4 is shown in the table, which gives the percentage of positive cells and their mean fluorescence intensity (MFI) and viability (mean ± standard deviation). Data shown are representative of at least three separate experiments.