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. Author manuscript; available in PMC: 2010 Aug 2.
Published in final edited form as: J Cell Biochem. 2009 Jun 1;107(3):473–481. doi: 10.1002/jcb.22145

Fig. 3.

Fig. 3

Polysome formation is disrupted by pathway inhibitors. C4-2B cells were treated with the indicated drug for 24 h with IGF-1 and cell lysates were layered over a 15–45% sucrose gradient and centrifuged. A: Half milliliters fractions were collected and the RNA isolated from each fraction was analyzed on an agarose gel to identify 18S and 28S rRNA. The triangle below the gels indicate the where the light and heavy fractions are found. B: Densitometry of each fraction was determined and the amount of 18S and 28S rRNA was expressed as a percentage of the total in all fractions. This was graphed as a function of percentage sucrose in each fraction and the curves were subjected to a 9 point smoothening. The arrows correspond to the increase in the pre-initiation complexes after LY294002 treatment and the corresponding decrease in heavy polysomes.