TABLE I.

List of Primers and Gene Products Used for RT-PCR

Gene product	Primer sequence	Annealing temperature (°C)	Product size (bp)
Cyclin D for [Cozar-Castellano et al., 2004]	ATGGAACACCAGCTCCTGTGCTGC	65	888
Cyclin D rev [Cozar-Castellano et al., 2004]	TCAGATGTCCACGTCCCGCACGT
EF1α2 fora	CAGTTCACCTCCCAGGTCAT	55	156
EF1α2 rev	GTTGTCCTCCACTTCTTGC
L32 for	AGGCATTGACAACAGGGTTC	55	130
L32 rev	GACGTTGTGGACCAGGAACT
β-actin for	GATGAGATTGGCATGGCTT	55	100
β-actin rev	CACCTTCACCGTTCCAGTTT
VEGF for [Muir et al., 2006]	CCTCCGAAACCATGAACTTT	55	637
VEGF rev [Muir et al., 2006]	AGAGATCTGGTTCCCGAAAC
L29 for	TCTTCCGGTTCTAGGCGCTT	55	556
L29 rev	GTCCTCATGTTGGCAGAGAT
HIF1α for	CTCAAAGTCGGACAGCCTCA	55	460
HIF1α rev	CCCTGCAGTAGGTTTCTGCT

The primer sequence for each primer as well as the annealing temperature and product size are given for each primer pair used.

^aPrimers were made specifically for this project unless otherwise noted.