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. 2010 Aug;177(2):803–812. doi: 10.2353/ajpath.2010.091231

Figure 6.

Figure 6

Direct identification of the ASARM peptide by Western blot using MEPE-ASARM antibody after electrophoresis on a 16.5% acrylamide Tricine gel. Lane 1, molecular weight marker; lane 2, recombinant MEPE; lane 3, hypophosphatemic dentin (patient 4); lane 4, hypophosphatemic dentin (patient 6); lane 5, control permanent dentin; lane 6, hypophosphatemic dentin (patient 1); lane 7, hypophosphatemic dentin (patient 7). After a 30-minute transfer, a faint band is observed at approximately 4 kDa for patients 1, 4, and 6 as indicated by an arrow. In contrast, this band was not detected for patient 7 (well treated adult) and for control dentin.