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. 2010 Aug;177(2):686–697. doi: 10.2353/ajpath.2010.090998

Figure 4.

Figure 4

The location and activity of NFκB p65 in intestinal epithelial cells in VDR knockout mice. A: NF-κB p65 location in colon in normal mice (VDR+/+) and VDR−/− mice without any treatment. Arrows indicate the p65 nuclear location (green). B: Western blot analysis of p65 levels in cytosolic (C) and nuclear (N) extracts isolated from VDR+/+ and VDR−/− mouse colons. C: The p65 level in cytosolic and nuclear extracts isolated from VDR+/− and VDR−/− MEFs. The nuclear protein Sp1, which is absent in the cytosolic fraction, serves as a nuclear protein loading control. D: Higher phospho-p65 level in the VDR−/− MEFs without any treatment. *P < 0.05. E: Increased phospho-p65 level in the VDR−/− mouse colonic epithelial cells without any treatment. Relative phospho-p65 band intensity in normal and VDR−/− mice is shown. Villin is used as a marker for epithelial cells and an internal control. β-actin is also used as an internal control. The mean ± SD is from three repeats. **P < 0.01.