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. 2010 Aug;177(2):908–917. doi: 10.2353/ajpath.2010.091150

Figure 2.

Figure 2

miRNAs are required for full SM differentiation. A: Brightfield images of hMSCs transfected with scramble (SCR) (left) or siDicer (right) oligonucleotides (oligo) at t = 3 weeks (×100 magnification) B: Western blot analysis of Dicer, CD105, calponin, SM22α, and ASMA at different time points of SM differentiation, upon Dicer silencing. Actin or tubulin is used as loading control. Numbers on the right indicate the level of reduction of protein levels at t = 3 weeks compared with the corresponding scramble control, after normalization to actin or tubulin. C: FACS analysis of CD105 and ASMA expression in SCR- and siDicer-transfected cells at t = 3 weeks, and hMSC (t = 0) used as control. Control: isotype control. D: qRT-PCR of SM-MHC in hMSCs transfected with scramble oligo or siDicer. Data were normalized to GAPDH expression. Error bars represent standard deviation (SD) between two experimental replicates. FC indicates fold change.