Figure 8.

A: Structure of NPR-B and nucleotide sequences of Npr2 in wild-type (+/+) and slw/slw mice. A 7-base deletion was identified in exon 8 of the slw/slw mice (“cggatcg” in red). Exon 8 of Npr2 codes for the domain just below the transmembrane domain of NPR-B. The deletion caused a frameshift and the appearance of a premature termination codon at codon 513 (“tga” in red). B: Detection of an Npr2 mutation by electrophoresis using acrylamide gel. Examination of the amplified PCR fragments including the 7-base deletion in exon 8 confirmed that the deletion was unique to the mutant allele.