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. 2010 Aug;177(2):1004–1016. doi: 10.2353/ajpath.2010.090703

Figure 1.

Figure 1

Ectopic expression of podoplanin increases motility of MCF7 breast carcinoma cells. A: Relative human podoplanin mRNA levels, measured by real-time RT-PCR in podoplanin-transfected (PDPN) and control (CTRL) MCF7 cells. Data represent mean ± SD (n = 3), with the value for CTRL set to 1. B: Immunoblot detection of human podoplanin in lysates of PDPN and CTRL MCF7 cells. 12 μg of total protein were used for each sample and β-actin served as control for equal loading. C: Closure of a scratch wound inflicted on monolayers of PDPN and CTRL MCF7 cells. The area closed at t = 48 hours is shown in % of the initial area at t = 0. Data represent mean ± SEM (n = 7). **P = 0.0013. D: Immunoblot detection of GTP-bound RhoA (from 1.5 mg of total protein) and total RhoA (in 30 μg of total protein) in lysates of PDPN and CTRL MCF7 cells. β-actin served as control for equal loading.