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. 2010 Aug;177(2):1004–1016. doi: 10.2353/ajpath.2010.090703

Figure 7.

Figure 7

mRNA and protein levels of tenascin-C and villin-1 are increased in MCF7 breast carcinoma xenografts that overexpress podoplanin, compared with control tumors. A: Relative mouse tenascin-C mRNA levels, measured by real-time RT-PCR in MCF7 tumors derived from podoplanin-overexpressing (PDPN) or control (CTRL) cell clones. Data are represented as mean ± SD (n = 3), with the value for clone CTRL 9 set to 1. B: Immunohistochemical detection of tenascin-C in cross-sections of PDPN and CTRL MCF7 tumor xenografts. C: Relative human villin-1 mRNA levels, measured by real-time RT-PCR as in A. D: Immunoblot detection of human villin-1 and human podoplanin in lysates of PDPN and CTRL MCF7 tumors. 180 μg (top) or 30 μg (middle and bottom) total protein were used for each sample and β-actin served as control for equal loading. E: Immunohistochemical detection of human villin-1 in cross-sections of PDPN and CTRL MCF7 tumor xenografts, with villin-1–positive tumor cells preferentially located near tumor stroma interfaces (dashed lines). Scale bars = 100 μm.