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Histone H3 modification profile. A: Results of PCR amplification of the ChIP products from seven gastric cancer cell lines, after precipitating the sonicated lysate with antibodies against modified histone H3: Met lys27, anti-trimethyl-H3 (lysine 27); Met lys9, anti-trimethyl-H3 (lysine 9); Ac lys9, anti-acetyl-H3 (lysine 9); and Ac H3, anti-acetyl-H3. B: Results of PCR amplification of the ChIP products from paired gastric cancer and nonneoplastic mucosa.
