Figure 5.

Quantification of leukocytes associated with remodeling arterioles and unremodeled arterioles from PDCs and decidua-only controls. Black bars: Decidua-only control culture arterioles (day 3: n = 20, day 6: n = 25). Striped bars: Unremodeled arterioles from PDCs (day 3: n = 28, day 6: n = 22). White bars: Actively remodeling arterioles from PDCs (day 3: n = 31, day 6: n = 27). Leukocyte infiltration was assessed by area of CD45, CD56, or CD68 staining and expressed as a ratio of the concentric ring areas 0–15 μm and 15–30 μm from arteriole lumens. A significant increase in CD45⁺ leukocyte/concentric ring area was observed at the 0–15 μm distance on both days 3 (A) and 6 (B). A significant increase in the proportion of uNK cells in the 0–15 μm ring surrounding actively remodeling vessels was observed at day 3 (C) and 6 (D). A significant increase in the proportion of macrophages within the 0–15 μm ring surrounding actively remodeling vessels was observed at both day 3 (E) and 6 (F). In contrast to day 3, a significant increase in the proportion of macrophages within the 15–30 μm zone around actively remodeling arterioles was observed at day 6 (F). *P < 0.05, **P < 0.01, ***P < 0.001.