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. 2010 Aug;177(2):865–872. doi: 10.2353/ajpath.2010.091096

Figure 4.

Figure 4

Kinetic analysis of epidermal cell proliferation (BrdU incorporation) after 0.5 BED of UVB. A: BrdU staining of nonirradiated human (Caucasian) regenerated skin. B: BrdU staining of 0.5 BED-irradiated human (Caucasian) regenerated skin 24 hours after irradiation. C: BrdU staining of 0.5 BED-irradiated human (Caucasian) regenerated skin 96 hours after irradiation. Scale bar = 100 μm. D: Quantitative analysis of BrdU incorporation 24 hours and 96 hours after 0.5 BED irradiation. Open bars: Caucasian-derived skins. Shaded bars: African-derived skins. *P < 0.05; **P < 0.01. E and F: Double immunofluorescence staining for Keratin K5 (green) and PCNA (red) in Caucasian (E) and African (F)-derived skins 24 hours after 0.5 BED irradiation. Nuclei are counterstained with DAPI (blue).