Figure 3.

Inhibition of NF-κB in MHV68 infection does not impair virus replication or inflammation during the acute period of infection in IFNγR^−/− mice. A: Acute replication in the lungs of IFNγR^−/− mice. On the indicated days post infection, lungs were harvested, disrupted, and titered on NIH 3T12 cells. The data were compiled from one or two experiments with five to ten mice analyzed per experiment. Data are shown as log₁₀ titer, and the bar indicates the geometric mean titer. The dashed line indicates the limit of detection of this assay as log₁₀1.7 or 50 pfu/ml of sample homogenate. B: Multistep growth curve in RLE-6TN cells infected with an MOI of 0.01 pfu per cell. Samples were harvested at the indicated time points and titers were determined on NIH3T12 cells. C–F: H&E staining of lung sections from mice infected with MHV68-MR, at 4, 9, and 12 dpi, respectively. Severe inflammation is noted in subpleural areas, around small and medium blood vessels, and surrounding airways by 12 dpi. Inset in E is shown at higher power view in F. G–J: H&E staining of lung sections from mice infected with MHV68- IκBαM, at 4, 9, and 12 dpi, respectively. Inset in I is shown at higher magnification in J. Magnification, ×10 (C–E, G–I).