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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2010 May 15;49(3):487–492. doi: 10.1016/j.freeradbiomed.2010.05.007

Fig. 4. The effect of mithramycin A on Sp1 and AhR expression, and Sp1 binding to the AhR promoter.

Fig. 4

Fig. 4

Fig. 4

MAECs obtained from hSod1Tg, hCatTg, hSod+Cat mice and their wild-type littermates were treated with the indicated concentrations of mithramycin A for 24 h. The Sp1 mRNA level (A) was determined by quantitative real-time RT-PCR and expressed relative to the GAPDH mRNA level. The AhR and Sp1 protein levels (B-D) were determined by western blot analysis and expressed relative to the immunoblot intensity of β-actin. The amount of Sp1 bound to the AhR promoter (E) was determined using ChIP analysis, and expressed as the ratio of input controls. Values represent means ± SEM of five separate experiments in which MAECs were pooled from four mice. *P<0.05 vs. wild-type cells under the same culture conditions; †P<0.05 vs. the same genotype cells without mithramycin A treatment.