Aegyptin inhibits collagen, but not convulxin, interaction with GPVI. (A) Collagen type I was injected at different concentrations (in μg/ml: a, 50; b, 25, c, 12.5; d, 6.25; and e, 3.175) over immobilized GPVI. (B) Collagen type I was incubated with buffer (sensorgram a) or 500 nM aegyptin (sensorgrams c–g) at the following concentrations (in μg/ml: a and c, 50; d, 25, e, 12.5; f, 6.25; and g, 3.175). The mixtures were injected over immobilized GPVI. Sensorgram b shows that aegyptin at 500 nM does not bind to immobilized GPVI. (C) Convulxin was injected at different concentrations (a, 10 nM; b, 5 nM and c, 2.5 nM) over immobilized GPVI. (D) Convulxin (a, 10 nM; b, 5 nM and c, 2.5 nM) was saturated with 500 nM of aegyptin and the mixture injected over immobilized GPVI (n = 3).