Table 1.
MMA processing schedule
Solution | Time in solution | Temp |
---|---|---|
Fixative (70% EtOH) | 60 h | 4°C |
70% acetone | 24 h | 4°C |
90% acetone | 1 h | 4°C |
100% acetone | 1 h | 4°C |
100% acetone | 1 h | 4°C |
85% MMA infiltration solution (vacuum desiccator) | 48 h | 4°C |
MMA embedding solution (waterbath in radiant oven) | 48 h | 37°C |