Table 1.
Effects of Hfx. volcanii agl deletions.
| Gene | Role | Effect1 of deletion on: | ||||||
|---|---|---|---|---|---|---|---|---|
| Hfx. volcanii Growth in high salt | S-layer Assembly | Shedding | Susceptibility to protease | S-layer glycoprotein SDS-PAGE migration | N-linked glycan structure | Reference | ||
| aglB | OTase2 | Decreased | No effect | Increased | No effect | Increased | No glycan | [114, 123] |
| aglD | GTase3 (sugar 5) | Decreased | Perturbed | Decreased | Decreased | Increased | Tetrasaccharide | [114, 123] |
| aglE | GTase (sugar 4) | No effect | n.d. | n.d. | No effect | No effect | Trisaccharide | [119] |
| aglF | Glucose-1-P uridyltransferase (sugar 3) | n.d.4 | n.d. | n.d. | Increased | Increased | Disaccharide | [120] |
| aglG | GTase (sugar 2) | n.d. | n.d. | n.d. | Increased | Increased | Monosaccharide | [120] |
| aglI | GTase (sugar 3) | n.d. | n.d. | n.d. | Increased | Increased | Disaccharide | [120] |
| aglM | UDP-glucose dehydrogenase (sugars 2, 3 (4?)) | n.d. | n.d. | n.d. | Increased | Increased | Monosaccharide | [122] |
| aglP | Methyltransferase (sugar 4) | n.d. | n.d. | n.d. | Increased | n.d. | Modified tetrasaccharide | [124] |
1Relative to level detected in parent strain; increased, decreased or no effect.
2OTase: oligosaccharyltransferase.
3GTase: glycosyltransferase.
4n.d.: not determined.