Table 2.
Effects of M. maripaludis and M. voltae agl deletions.
| Gene | Role | Effect1 of deletion on: | ||||
|---|---|---|---|---|---|---|
| Cell flagellation | Motility | Flagellin SDS-PAGE migration | N-linked glycan structure | Reference | ||
| M. maripaludis | ||||||
| aglA | GTase2 (sugar 3) | Present | Decreased | Increased | Disaccharide | [116] |
| aglB | OTase3 | Absent | Non-motile | Increased | No glycan | [116] |
| aglL | GTase (sugar 4) | Present | Decreased | Increased | Modified trisaccharide | [116] |
| aglO | GTase (sugar 2) | Absent | Non-motile | Increased | Monosaccharide | [116] |
| MMP0350 | Acetyltransferase (sugar 2) | Absent | Non-motile | Increased | Monosaccharide | [117] |
| MMP1081 | Acetamidino transfer (sugar 3) | Absent | Decreased | Increased | Modified trisaccharide | unp |
| MMP1085 | Methyltransferase (sugar 4) | Present | n.d. | Increased | Modified tetrasaccharide | unp |
|
| ||||||
| M. voltae | ||||||
| aglA | GTase (sugar 3) | Present | n.d. | Increased | Disaccharide | [115] |
| aglB | OTase | Absent | Non-motile | Increased | No glycan | [115] |
| aglC | GTase (sugar 2) | Absent | n.d. | Increased | Monosaccharide | [125] |
| aglK | GTase (sugar 2) | Absent | n.d. | Increased | Monosaccharide | [125] |
N. B.: M. maripaludis wild type N-linked glycan is a tetrasaccharide, M. voltae wild type N-linked glycan is a trisaccharide.
1Relative to level detected in parent strain.
2GTase: glycosyltransferase.
3OTase: oligosaccharyltransferase.
4n.d.: not determined.
5unp: unpublished data.