Fig. 8.

Immunoreactivity of gp91 phox and p47 phox, subunits of NADPH oxidase in the neuronal cultures treated with BSO, an inducer of oxidative stress; the PDE2 inhibitor Bay 60-7550 (Bay) and the NADPH oxidase inhibitor apocynin reversed the BSO-induced increase in the immunoreactivity of gp91 phox and p47 phox subunits. The PKG inhibitor KT-5823 markedly blocked the Bay 60-7550-induced effect, increasing gp91 phox and p47 phox immunoreactivity. The PKA inhibitor H89 had little effect. Representative immunofluorescent photomicrographs of gp91 phox (A–F; green staining) and p47 phox immunostaining (G–L; red staining) in neuronal cultures are shown (magnification, 200×).