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. Author manuscript; available in PMC: 2010 Aug 2.
Published in final edited form as: Mol Cell. 2007 Mar 23;25(6):889–901. doi: 10.1016/j.molcel.2007.02.014

Figure 4. Hyperactivation of Crz1PVIVIT Requires Ca2+ and Calcineurin Activity.

Figure 4

(A) β-galactosidase activity with or without CaC12 addition was determined for cells containing 4×-CDRE-lacZ and expressing Crz1 or Crz1PVIVIT, either alone or cotransformed with the multicopy plasmid pKC60 expressing PMC1. Error bars indicate standard deviation (see Experimental Procedures for details).

(B) β-galactosidase activity was determined from strains with the indicated genotype that also contained 4×-CDRE-lacZ and were transformed with plasmids expressing either CRZ1 (white bars) or Crz1PVIVIT (black bars). Cells were grown in selective media to retain the respective plasmids. Error bars indicate standard deviation (see Experimental Procedures for details).

(C) Immunoblot analysis of strains, with the indicated genotype, transformed with plasmids expressing either HA-tagged Crz1 or Crz1PVIVIT, using anti-HA antibody. Pgk1 is used as a loading control.