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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Immunol. 2009 Aug 26;183(6):3955–3962. doi: 10.4049/jimmunol.0804103

FIGURE 8.

FIGURE 8

Effect of p38 and ERK inhibitors on IL-12-mediated restoration of MLN T cell ATF-2 phosphorylation following EtOH intoxication and burn injury. MLN T cells (1 × 107 cells/ml) harvested from various experimental groups were pretreated with SB203580 (10 μM) and/or PD98059 (50 μM) for 30 min and then with rIL-12 (10 ng/ml) 1 h before their stimulation with anti-CD3 Abs (1 μg/ml) for 5 min and lysed. Lysates were analyzed for ATF-2 phosphorylation by Western blot. Blots were stripped and reprobed for β-actin contents in various lanes. Blots obtained from four animals in each group were analyzed using densitometry. Densitometric values for ATF-2 phosphorylation were normalized to β-actin and are shown as means ± SEM. *, p < 0.05 compared with saline plus shams and CD3 plus IL-12 group; #, p < 0.05 compared with respective CD3 plus IL-12 group.