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. Author manuscript; available in PMC: 2010 Aug 2.
Published in final edited form as: J Biomol Screen. 2009 Jul 29;14(7):853–858. doi: 10.1177/1087057109340314

Fig 1.

Fig 1

Screen for the identification of senescence in cancer cells. A. Senescent morphology and SA-β-gal activity. Phase contrast microscopy of DU145 cells cultured with DMSO (control) or 250nM AZQ, identified by this study, for 3 days, fixed and stained for SA-β-gal activity overnight, as previously described(2). Original image magnification: 400x. B. Multi-step screening strategy. Prostate cancer cell lines were exposed to a library of compounds for 3 days, fixed and stained overnight for SA-β-gal activity, followed by staining with Hoechst 33342. Compounds of interest were initially identified by decreased Hoechst 33342 fluorescence indicative of low cell numbers. These wells were then visually assessed for the extent of SA-β-gal activity and senescent morphology.