Fig 2.

Development of Hoechst 33342 fluorescence to identify senescence in treated cancer cells. A. DU145 cells were cultured in a 96-well plate treated with increasing doses of Doxorubicin (n=14). Cells were cultured three days, fixed, stained for SA-β-gal activity and Hoechst 33342. Blank wells were included as negative controls. B. Calculation of Z′ in senescent versus proliferating DU145 cells. Cells were cultured with 25nM Doxorubicin (DOX) or untreated (UN) for 3 days before being stained and Hoechst 33342 fluorescence measured by plate reader. The average Z′ of all four experiments was 0.53 (p<0.003). Error bars represent standard error.