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. Author manuscript; available in PMC: 2010 Aug 2.
Published in final edited form as: Biochemistry. 2006 Aug 1;45(30):9074–9084. doi: 10.1021/bi060567d

Figure 1.

Figure 1

Fluorescence spectra of mant-ATP in the absence and presence of wild-type PKR. A: mant-ATP (), PKR (.....), mant-ATP + PKR (Inline graphic, blue) and mant-ATP +PKR + unlabeled ATP (Inline graphic, red). Spectra were recorded at an excitation wavelength of 295 nm with excitation and emission bandwidths of 2 nm. B: Spectra of mant-ATP (), and mant-ATP + PKR (Inline graphic, red). Spectra were recorded at excitation wavelength of 360 nm with excitation and emission bandwidths of 2 nm. Concentrations: mant-ATP, 122 μM; PKR, 1 μM; ATP, 2 mM.