Figure 1.
Fluorescence spectra of mant-ATP in the absence and presence of wild-type PKR. A: mant-ATP (—), PKR (.....), mant-ATP + PKR (
, blue) and mant-ATP +PKR + unlabeled ATP (
, red). Spectra were recorded at an excitation wavelength of 295 nm with excitation and emission bandwidths of 2 nm. B: Spectra of mant-ATP (—), and mant-ATP + PKR (
, red). Spectra were recorded at excitation wavelength of 360 nm with excitation and emission bandwidths of 2 nm. Concentrations: mant-ATP, 122 μM; PKR, 1 μM; ATP, 2 mM.
