PRL-1 regulates cell adhesion. A, cell lysates (40 μg) were analyzed by Western blotting in epithelial markers, E-cadherin, vinculin, and paxillin. GAPDH was used as a loading control. B, relative expression is average fold mean ± S.D. The protein level changes from three independent experiments normalized to vector. ∗, P < 0.05; ∗∗, P < 0.01, compared with vector control.