Fig. 6.

Inhibition of Na⁺-independent E217G initial uptake in suspended rat hepatocytes by S-sulfone (A) and S-sulfide (B). Na⁺-independent [³H]E217G initial uptake (1 μM, 60 nCi/ml, 90 s) was determined in Na⁺-free buffer in the presence of increasing concentrations of S-sulfone (0.5–100 μM) and S-sulfide (0.5–100 μM). Symbols and error bars represent the mean ± S.D. of n = 3 livers in duplicate. The dotted curve represents the best fit of the sigmoidal inhibitory E_max model to the data by using WinNonlin; the curve shape factor (r) was estimated as 0.8 ± 0.2 and 1 ± 0.2 for S-sulfone and S-sulfide, respectively.