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. 2010 Aug;334(2):430–438. doi: 10.1124/jpet.110.167544

Fig. 6.

Fig. 6.

GSIS, ATP levels, and UCP2 expression in Ephx2(−/−) and Ephx2(+/+) islets. A, Epxh2(+/+) and Epxh2(−/−) islets were treated with 3 mM glucose, 25 mM glucose, or 30 mM KCl plus 250 μM diazoxide plus 3 mM glucose. Insulin concentrations in the media and islet pellets were determined using an insulin ELISA kit. The data shown are based on the results of five experiments. B, islets were isolated from Epxh2(+/+) mice incubated with either t-AUCB (100 μM) or vehicle for 12 h and then incubated with 3 mM glucose, 25 mM glucose, or 30 mM KCl plus 250 μM diazoxide plus 3 mM glucose. Insulin concentrations in the media and islet pellets were determined with an insulin ELISA kit. The data shown are based on the results of four experiments. C, ATP levels. After overnight culture, Ephx2(−/−) or Ephx2(+/+) islets were incubated with 3 or 25 mM glucose and then assessed for concentrations of ATP (n = 3). D, Western blot analysis of UCP2 in Ephx2(−/−) and Ephx2(+/+) islets. ∗, P < 0.05; ∗∗, P < 0.01 versus Ephx2(+/+) incubated with vehicle.