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. Author manuscript; available in PMC: 2010 Aug 2.
Published in final edited form as: Science. 2010 Feb 4;327(5968):996–1000. doi: 10.1126/science.1184208

Fig. 3.

Fig. 3

MRG15 modulates alternative splicing. (A and B) Chromatin immunoprecipitation of MRG15 along FGFR2 (A) and CD44 (B) in PNT2 (red) and hMSC (black) cells. Input was normalized to unmodified H3. The RPL13a promoter was a positive control. Values represent means ± SEM from three to six independent experiments. (C to F) Quantitative RT-PCR analysis of exon ratios after overexpression of EGFP-MRG15 [(C) and (D), MRG15] or down-regulation of both MRG15 and MRGX [(E) and (F), siMRG] in PNT2 (red) or hMSC (black) cells. Ratios are as follows: exon IIIb/IIIc for FGFR2 and exon v6/e2 for CD44. Values represent means ± SEM of percentages relative to EGFP or siCyclophilin B used as controls from five independent experiments. *P<0.05, **P<0.01, Student's t test compared to control.