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. Author manuscript; available in PMC: 2010 Aug 2.
Published in final edited form as: Science. 2010 Feb 4;327(5968):996–1000. doi: 10.1126/science.1184208

Fig. 4.

Fig. 4

H3-K36me3 recruits PTB to exon IIIb via MRG15. (A) Immunoblot detection of PTB and MRG15 after immuno-precipitation of PTB. (B) Reciprocal coimmunoprecipitation of over-expressed MRG15-V5/His and PTB-HA in Hela cells. (C to E) RNA immunoprecipitation of PTB, MRG15, or Sm proteins in FGFR2 exons IIIb and IIIc in PNT2 (red) or hMSC (black) cells. Values represent means ± SEM from three independent experiments. (F and G) Overexpression of MRG15 (F) or SET2 (G) in PNT2 (red) or hMSC (black) cells for 48 hours before RNA immunoprecipitation of PTB. Values represent means ± SEM from three independent experiments. (H and I) Quantitative RT-PCR analysis of exon ratios in PNT2 cells depleted of MRG15 and MRGX (siMRG, yellow) or cyclophilin B (siCycB, red) after overexpression of SET2 or EGFP. Ratios are as follows: exon IIIb/IIIc for FGFR2 (H) and exon v6/e2 for CD44 (I). Values represent means T SEM from five independent experiments. *P < 0.05, **P < 0.01, Student's t test compared to control.