Figure 1. Analysis of p27 IRES-mediated translation using a new bioluminescent mouse model reveal robust translational control in the pituitary, which is reduced in DKC1^m background.

A) Schematic of the dicistronic transgene used to generate bioluminescent p27 IRES^T animals. B) Analysis of p27 IRES^T mice. Graph is mean ± SEM of percentage p27 IRES activity (FLuc values) measured in different organs prepared from three p27 IRES^T mice and normalized by the mRNA dicistronic expression. C) p27 IRES-mediated translation is markedly reduced in DKC1^m pituitary glands. Levels of p27 IRES-mediated translation were measured in pituitary glands isolated from p27 IRES^T and p27 IRES^T;DKC1^m mice. Columns, mean ± SEM of ratio FLuc/RLuc (FL/RL) measured in four pairs of age-matched animals; bars, SEM. Statistical analysis was carried out using Student t test. *P<0.05.