Figure 5. Impaired rRNA pseudouridylation is associated with reductions of p27 protein levels but not of the telomerase RNA component (TERC) in the DKC1^S485G mutant tumor.

A) Chromatographs of HPLC analysis of the 18S rRNA pseudouridylation are shown. Bar graph shows quantification of the Ψ/C ratio in samples from normal pituitary and the DKC1^S485G mutant tumor. B) The levels of the TERC were measured by QPCR in two normal pituitaries and the DKC1^S485G pituitary tumor. Analysis was performed in triplicate on two different samples per specimen. No significant differences between the samples were found. P>0.05 was determined employing the unpaired two-tailed t test. C) p27 protein levels are markedly reduced in the tumor carrying the DKC1^S485G mutation. Western blot (left) and QPCR analysis (right) of p27 expression was performed using protein extracts and total RNA prepared from a normal pituitary and the tumor carrying the DKC1^S485G mutantion. Densitometric analysis of p27 over β-actin is shown (bottom left). No significant differences in p27 mRNA levels were measured between the samples. P>0.05 was determined employing the unpaired two-tailed t test.