Figure 1. Mice genetically lacking the ability to secrete cholesterol into bile have normal fecal sterol loss.
Wild type mice (WT) or littermates with moderate (NPC1L1-Tg-Mod) or high level (NPC1L1-Tg-High) overexpression of hepatic NPC1L1 were fed diets containing 0.015% or 0.2% cholesterol (wt/wt) for six weeks.
(A) The concentration of cholesterol in gall bladder bile was determined by gas liquid chromatography.
(B) Fecal neutral sterol excretion was determined by gas liquid chromatography.
(C) Fractional cholesterol absorption was determined using the dual fecal isotope method. Data represent the means ± SEM from 4–7 mice per group, ** = significantly different than WT within each diet group, P < 0.01.