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. Author manuscript; available in PMC: 2010 Aug 2.
Published in final edited form as: Pathol Lab Med Int. 2009 Aug 1;1:7–12. doi: 10.2147/plmi.s6249

Figure 1.

Figure 1

IL-1 inhibits ZBP-89 mRNA expression in HGF. HGF cultures were serum-deprived for 16 hours prior to addition of IL-1β (10 ng/ml) and/or IL-4 (10 ng/ml). Total RNA was harvested 12 hours after cytokine treatment, quantitated by real-time PCR and normalized to levels of GAPDH.

Notes: The graph represents data from seven independent experiments performed in triplicate, expressed as mean ± SEM. Statistical significance was determined using student’s t-test. **p < 0.01.

Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HGF, human gingival fibroblasts; IL, interleukin; PCR, polymerase chain reaction; SEM, standard error of mean.