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. Author manuscript; available in PMC: 2010 Aug 2.
Published in final edited form as: Pathol Lab Med Int. 2009 Aug 1;1:7–12. doi: 10.2147/plmi.s6249

Figure 4.

Figure 4

ZBP-89 mRNA is inhibited by IL-1β and LPS, but not by TNF-α in WI-38 cells. WI-38 cell cultures were serum-deprived for 16 hours prior to addition of cytokines/inflammatory mediator (10 ng/ml). Total RNA was harvested 12 hours after treatment, quantitated by real-time PCR, and normalized to levels of GAPDH.

Notes: The graph represents data from two independent experiments performed in triplicate, expressed as mean ± SEM.

Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; EGF, epidermal growth factor; IL, interleukin; LPS, lipopolysaccharide; PCR, polymerase chain reaction; TNF, tumor necrosis factor; SEM, standard error of mean.