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. Author manuscript; available in PMC: 2011 Jul 23.
Published in final edited form as: Brain Res. 2010 May 7;1345:176–181. doi: 10.1016/j.brainres.2010.04.074

Figure 4. Effects of 17β-estradiol and ICI 182,780 on GSK3β in OA treated SH-SY5Y cells.

Figure 4

Human neuroblastoma SH-SY5Y cells were treated with OA (100 nM) in the presence of absence of 17β-estradiol (E2, 10 μM) and ICI 182,780 (1 μM) for three hours. Total GSK3β and p-GSK3β (Ser 9) were assessed via western blot analysis. OA, E2 and/or ICI 182,780 were dissolved in 0.1% DMSO. Vehicle control was treated with 0.1% DMSO as vehicle. All data are normalized to total GSK3β and are expressed as a percentage of control. Data are presented in mean ± SEM for n=4. # <0.05 compared to control and * <0.05 compared to OA only. Groups connected by the line were different at p<0.05.