TABLE 3. Role of the thyroid axis in TRH antagonism of ethanol.

Hypophysectomized mice received 4.3 g/kg, intact mice 4.7 g/kg of ethanol. All drugs were administered intraperitoneally. Hypophysectomized mice used in this study had pituitaries removed at Charles River Breeding Laboratories 3 days prior to the test. The effectiveness of the procedure was confirmed by holding mice for an additional month to establish that further weight gain did not occur. At that time hypophysectomized mice weighed 20 g and unoperated controls of equal weight at the time of surgery weighed 35 g. Temperature was recorded 40 minutes after ethanol treatment.

Treatment	N	Sleeping Time	Rectal Temperature
		min ± S.E.M.	°C ± S.E.M.
Hypophysectomized mice
Saline	9	58.8 ± 9.60	32.9 ± 0.39
TRH, 10 mg/kg	10	31.2 ± 2.75b	34.0 ± 0.27a
Intact mice
Saline	9	75.9 ± 5.36	36.3 ± 0.09
Triiodothyronine 1 mg/kg	10	75.9 ± 13.15	36.5 ± 0.19
Thyrotropin, 10 mg/kg	10	79.2 ± 8.85	36.5 ± 0.19
TRH, 10 mg/kg	10	44.0 ± 394c	37.0 ± 0.10c

When compared with saline:

^aP < .05.

^bP < .02.

^cP < .001.